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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Int J Cancer. 2013 Oct 15;134(7):1758–1766. doi: 10.1002/ijc.28499

Fig. 5. Ex vivo analysis of tumor cell death following treatment with Dox and MN-siBIRC5 in BT-20 tumor xenografts.

Fig. 5

(A) Detection of caspase-3 activity in tumor lysates. Excised tumors were analyzed by caspase-3 enzyme assay. Data are represented as ± S.D. (n=8, two-tailed Student’s t-test, ***P<0.0005 compared to PBS control). Caspase-3 activation was significantly higher in the Dox-MN-siBIRC5 treatment group relative to PBS. (B) Detection of cleaved PARP fragments in tumor tissue sections by immunofluorescence. Nuclei stained with (DAPI, Blue) and cleaved PARP immunostained (Texas red, Red) were visualized by fluorescence microscopy. PARP cleavage was most prominent in the Dox-MN-siBIRC5 treatment group.