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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Structure. 2013 Nov 7;22(1):82–93. doi: 10.1016/j.str.2013.09.019

Figure 4. Position-specific peptide arrays reveal modulator preferences for CALP.

Figure 4

SubAna arrays of 10 peptides are clustered by residue position, with each row labeled according to the background sequence of the source array (see key for sequence information). The WT residues are listed to the right of each row. Residues in the initial sequence are substituted by all 20 L-amino acids, as indicated at top (see text). The motif positions P0 and P−2 reveal clear preferences for only a few residues. Positive modulator preferences are seen as consistently stronger binding than WT following substitution of e.g., Arg at P−1 or Trp at P−5, independent of the background peptide sequence. Negative preferences are also seen as consistently weaker binding compared to WT, e.g., following substitution of Gly at P−1 or Asp at P−3. See also Figure S5.