TABLE 2. Calorimetric data for KyoT2 binding to CSL.
Macromolecule | KyoT2 Ligand |
K (M−1) |
Kd
(μM) |
ΔG°
(kcal/mol) |
ΔH°
(kcal/mol) |
−TΔS°
(kcal/mol) |
---|---|---|---|---|---|---|
CSL | 184 - 210 | 8.5 ± 2.3 × 107 | 0.012 | −10.8 ± 0.2 | −15.5 ± 0.7 | 4.7 ± 0.5 |
CSL + DNA | 184 - 210 | 1.1 ± 0.4 × 108 | 0.011 | −10.9 ± 0.3 | −11.0 ± 0.6 | 0.03 ± 0.3 |
BTD | 184 - 210 | 4.0 ± 0.8 × 107 | 0.026 | −10.4 ± 0.1 | −13.6 ± 0.4 | 3.3 ± 0.5 |
CSL | 184 - 200 | 3.1 ± 0.7 × 107 | 0.033 | −10.2 ± 0.1 | −13.1 ± 0.6 | 2.9 ± 0.5 |
CSL | 182 - 210 | 7.1 ± 1.5 × 107 | 0.015 | −10.7 ± 0.1 | −16.0 ± 1.0 | 5.2 ± 0.9 |
CSL | 182 - 196 | 1.0 ± 0.8 × 108 | 0.013 | −10.9 ± 0.1 | −10.8 ± 0.6 | −0.1 ± 0.6 |
All experiments were performed at 25°C. Values are the mean of at least three independent experiments and errors represent the standard deviation (S.D.) of multiple experiments. See also Table S2 for ITC data of minimal KyoT2 peptide sequence that binds CSL.