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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Structure. 2013 Nov 27;22(1):70–81. doi: 10.1016/j.str.2013.10.010

TABLE 2. Calorimetric data for KyoT2 binding to CSL.

Macromolecule KyoT2
Ligand		 K (M−1) Kd
(μM) 		ΔG°
(kcal/mol) 		ΔH°
(kcal/mol) 		−TΔS°
(kcal/mol)
CSL 184 - 210 8.5 ± 2.3 × 107 0.012 −10.8 ± 0.2 −15.5 ± 0.7 4.7 ± 0.5
CSL + DNA 184 - 210 1.1 ± 0.4 × 108 0.011 −10.9 ± 0.3 −11.0 ± 0.6 0.03 ± 0.3
BTD 184 - 210 4.0 ± 0.8 × 107 0.026 −10.4 ± 0.1 −13.6 ± 0.4 3.3 ± 0.5
CSL 184 - 200 3.1 ± 0.7 × 107 0.033 −10.2 ± 0.1 −13.1 ± 0.6 2.9 ± 0.5
CSL 182 - 210 7.1 ± 1.5 × 107 0.015 −10.7 ± 0.1 −16.0 ± 1.0 5.2 ± 0.9
CSL 182 - 196 1.0 ± 0.8 × 108 0.013 −10.9 ± 0.1 −10.8 ± 0.6 −0.1 ± 0.6

All experiments were performed at 25°C. Values are the mean of at least three independent experiments and errors represent the standard deviation (S.D.) of multiple experiments. See also Table S2 for ITC data of minimal KyoT2 peptide sequence that binds CSL.