Table 3. Temperature dependence of KyoT2 binding to CSL.
T (°C) | K (M−1) | Kd (μM) |
ΔG°
(kcal/mol) |
ΔH°
(kcal/mol) |
−TΔS°
(kcal/mol) |
|
---|---|---|---|---|---|---|
CSL + KyoT2 |
5 | 6.2 ± 1.4 × 107 | 0.017 | −9.9 ± 0.1 | −3.9 ± 0.4 | −6.0 ± 0.5 |
15 | 8.3 ± 2.3 × 107 | 0.013 | −10.4 ± 0.2 | −6.3 ± 0.3 | −4.2 ± 0.5 | |
25 | 9.4 ± 1.2 × 107 | 0.011 | −10.9 ± 0.1 | −14.6 ± 0.2 | 3.7 ± 0.2 | |
35 | 3.5 ± 0.6 × 107 | 0.029 | −10.6 ± 0.1 | −16.7 ± 0.7 | 6.1 ± 0.5 |
Values are the mean of at least three independent experiments and the errors represent the S.D. of multiple experiments.