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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Comb Chem High Throughput Screen. 2014 Jan;17(1):12–24. doi: 10.2174/13862073113169990056

Figure 4. Cellular proliferation assessment in the presence or absence of seven transfection reagents.

Figure 4

Figure 4

(A) Bar graph summary of the quantification of cellular proliferationbased on whole well imaging of Hoechst-stained nuclei and automated image analysis. Seven commercially available chemical transfection reagents are tested at 3 dilutions D1, D2 and D3 corresponding respectively to 0.025, 0.05 and 0.1 μL per well compared to control wells in absence of transfection reagent. Cell proliferation is measured at 24, 48 and 72 hours post transfectionwith chemical transfection reagent only and in combination with the EGFP DNA plasmid or scrambled siRNA duplex. (B) Bar graph summary of the quantification of TE based on whole well imaging and automated image analysis of Hoechst-stained nuclei and EGFP expression. TE is calculated as the percentage of EGFP-expressing cells as determined by the total count of EGFP masks overlapping with a Hoechst-stained nucleus mask compared to the total count of Hoechst-stained nuclei masks. Seven commercially available chemical transfection reagents are tested at 3 dilutions D1, D2 and D3 corresponding respectively to 0.025, 0.05 and 0.1 μL per well compared to control wells in absence of transfection reagent. TE is measured at 24, 48 and 72 hours post transfection with chemical transfection reagent only and in combination with the EGFP DNA plasmid or scrambled siRNA duplex.