Table 1.
Summary of the seven chemical transfection reagents used in this study.
Guidelines from the respective manufacturer are summarized for each reagent including the recommended amount based on volume to mass ratio or on well surface area, extrapolated to 384-well microtiter plate format. For each reagent, the tested range was within the recommended range.
| Transfection reagent volume | |||||||
|---|---|---|---|---|---|---|---|
| 96-well format | 384-well format | ||||||
| Recommended | Extrapolated | Tested | |||||
| Transfection reagent | Provider | Formulation | Recommended for DNA/siRNA | Based on volume:mass ratio (μL/μg DNA or siRNA) | Based on well surface area (μL) | For 20 ng DNA (μL) | |
| TransIT-LT1 | Mirus Bio | Non-liposomal lipid | DNA | 2:1 to 8:1 | - | 0.04 to 0.16 | 0.025 to 0.1 |
| X-tremeGENE 9 | Roche | Lipids and other | DNA/siRNA | 3:1 to 5:1 | - | 0.06 to 0.3 | |
| FuGENE 6 | Promega | Non-liposomal lipid | DNA | 1.5:1 to 6:1 | - | 0.03 to 0.12 | |
| FuGENE HD | Promega | Non-liposomal lipid | DNA | 1.5:1 to 4:1 | - | 0.03 to 0.08 | |
| Lipofectamine LTX | Invitrogen | Cationic lipid | DNA | 1:1 to 6:1 | - | 0.02 to 0.12 | |
| Lipofectamine RNAiMAX | Invitrogen | Cationic lipid | DNA/siRNA | - | 0.1 to 0.3 | 0.025 to 0.075 | |
| DharmaFECT 1 | Thermo-Scientific | Cationic lipid | siRNA | - | 0.05 to 0.5 | 0.01 to 0.1 | |