Effect of IL-3 on EOS binding to plexin C1. EOS were pre-activated or not with IL-3 (2 ng/ml) for 20 h. EOS were then seeded on the indicated protein, plexin C1 or VCAM 7d (positive control), coated at 10 μg/ml and blocked with gelatin. Percentage of EOS bound to coated protein was determined with the EPO assay. *indicates % adhesive EOS is higher than either EOS on gelatin alone after treatment with IL-3, or untreated cells on plexin C1 (P<0.05, one way analysis of variance, n=3). †indicates adhesion of untreated EOS to plexin C1 is higher than adhesion to gelatin (P<0.05, paired t test, n=3).