. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: Clin Immunol. 2013 Nov 23;150(1):90–100. doi: 10.1016/j.clim.2013.11.009

Table 1.

Eosinophils are a major source of sema7A mRNA in BAL cells after an allergen challenge^*

	Total cells × 10⁶	Mono/Mac (%)	Neut (%)	Lymph (%)	Eos (%)	Sema7A mRNA
Unfractionated BAL cells before SBP-Ag	11±2	92.5±2.9	0.8±0.4	6.3±2.6	0.5±0.1	1
Unfractionated BAL cells after SBP-Ag	238±43	16.3±5.1	1.9±0.8	6.4±3.7	75.7±3.7	163±84^†
Purified BAL EOS after SBP-Ag	not applicable	<1	<1	<1	>99	1617±865^‡

BAL cells were obtained before and 48 h after SBP-Ag. Total cell counts and differential cell counts for monocytes (Mono), macrophages (Macs), neutrophils (Neuts), and EOS are expressed as mean±SD. Sema7A mRNA was analyzed by qPCR in unfractionated BAL cells and purified BAL EOS. Sema7A mRNA was normalized to the housekeeping gene (GUSB) and fold change was calculated compared to sema7A mRNA level in unfractioned BAL cells before SBP-Ag. Sema7A mRNA fold change was log transformed and a student’s t test was performed. Data are expressed as mean ± SE,

^†

indicates statistical difference (p<0.05) compared to BAL cells before SBP-Ag, and

^‡

indicates difference (p<0.05) compared to total BAL cells after SBP-Ag, n=4.