Fig. 3.

Effect of desensitization of central TRPV1 receptors on forced swims in 26°C and 41°C water. Mice were pretreated i.t. with vehicle or RTX (0.25 µg). The next day, mice were injected with vehicle or a low dose of RTX (0.02 mg/kg s.c.) and tested 24 and 48 h later in a 15-min forced swims in water maintained at either 26°C or 41°C. The time spent immobile during the first 5 min of each swim was measured. Comparisons between different treatments were performed using one-way ANOVA followed by Tukey’s post-hoc analysis and marked with an asterisk (*) when different from each other. Comparisons between same treatments on different days were performed using a paired Students t-test and when significantly different indicated with a pound sign (#) on day 2 (panels B and D).