Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Am J Reprod Immunol. 2013 Dec 6;71(2):95–108. doi: 10.1111/aji.12182

Figure 1. Flow cytometry gating used in the analysis of endometrial T-cell phenotype and function.

Figure 1

Panel (A): For phenotypic analysis, after initial gating of endometrial lymphocytes and doublet discrimination, viable cells were gated for CD3+CD66b− (not shown) and then for CD4+ (top row) and CD8+ (bottom row) T-cells. The resulting populations were gated for CD45RA, CCR7, CD38, HLA-DR, CCR5 and CXCR4. Panel (B): For analysis of endometrial T-cell responses to polyclonal stimulation, seven functional responses (CD107a, IL-2, IL-10, IL-17, TNF-α, IFN-γ and MIP-1β) were measured. Initial gating was performed to identify lymphocytes and doublet discrimination (not shown), followed by gating of viable cells on CD4+ (top row) and CD8+ (bottom row) T-cells, and finally for individual responses.