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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Eur J Cancer. 2013 Oct 30;50(2):457–468. doi: 10.1016/j.ejca.2013.10.001

Figure 1. The c-Src inhibitor completely blocked E2-induced apoptosis after long-term treatment.

Figure 1

(A) The morphological changes after 8 weeks treatment with different combinations. MCF-7:5C cells were long-term treated with vehicle (0.1% EtOH), PP2 (5×10−6mol/L), E2 (10−9 mol/L), and E2 (10−9mol/L) plus PP2 (5×10−6mol/L) in T25 flasks, respectively. Cells were photographed under bright field illumination at (×20) magnification (Zeiss). (B) Cell-cycle changes after different treatments. Cells treated in different combinations were harvested and gradually fixed with 75% EtOH on ice. After staining with propidium iodide (PI), cells were analyzed through flow cytometry. All the data shown were representative of at least three separate experiments with similar results.