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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Eur J Cancer. 2013 Oct 30;50(2):457–468. doi: 10.1016/j.ejca.2013.10.001

Figure 3. The c-Src inhibitor collaborated with E2 to up-regulate ER target genes.

Figure 3

(A) ERE activity changes in different cells. Cells treated in different combinations were seeded in 24-well plates in triplicate and transfected with ERE firefly luciferase plasmid plus renilla luciferase plasmid as in Materials and Methods. (B) The pS2 mRNA expression. Different combinations treated cells were grown in 6-well plates in triplicate. The RNA was harvested in TRIzol for real-time PCR analysis. P<0.001, ** compared with control. (C) The PR mRNA expressed levels. The RNA of different cells was harvested in TRIzol for real-time PCR analysis. P<0.001, ** compared with control. (D) PR protein changes after long-term treatment. Cell lysates were harvested from different treated cells. PR was examined by immunoblotting. β-actin was detected for loading control. All the data shown were representative of at least three separate experiments with similar results.