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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Diabetologia. 2013 Nov 8;57(2):413–423. doi: 10.1007/s00125-013-3101-z

Fig. 5.

Fig. 5

HFD-induced TXNIP expression co-localises within retinal vasculature and glial cells. (a) Increased TXNIP expression was observed in the GCL, INL and, to a lesser extent, ONL in the W+F group relative to the control W group. (b, c) Negative controls (b, normal rabbit serum; c, no primary antibody) showed specific binding for TXNIP antibody in the GCL and INL but not in the ONL. (d) HFD induced GFAP activation in the W+F group. Co-localisation of TXNIP with glutamine synthase (GS) (e), GFAP (f) and isolectin-B4 (g) staining showed strong association in the GCL, suggesting astrocytes, Müller cell endfeet and retinal microvasculature in GCL (enlarged in h), as well as deep retinal capillaries in the INL. Magnification, ×200, scale bars, 25 μm