Fig. 7.

TXNIP is required for palmitate-induced IL-1β maturation in HRE cells. Representative blot and western blot analysis of cleaved IL-1β (a) in HRE cells transfected with either 0.6 μmol/l scrambled siRNA (SC group) or Txnip siRNA after incubation with PN alone, Pal-BSA alone or both (Pal-BSA+PN), in comparison with control BSA in the SC group. TXNIP knockdown resulted in abrogation of the Pal-BSA-mediated maturation of cleaved IL-1β. Protein levels were normalised to α-tubulin and SC BSA control. n=3 or 4; *p<0.05 vs SC BSA; ^†p<0.05 vs all SC groups. (b) IL-1β release into the HRE cell conditioned media was quantified using an IL-1β sensitive ELISA kit. n=3–4; *p<0.05 vs SC BSA; ^†p<0.05 vs SC PN-BSA, Txnip siRNA PN-BSA and Txnip siRNA Pal-BSA+PN; ^‡p<0.05 vs all other groups