Figure 6. PKCα but not integrin α3 is required for the regulation of motility through CD151free.
(A) Immunoblotting of Triton-X 100 lysates from HEp3 cells transfected with the control, integrin α3-specific or PKCα-specific siRNA to evaluate the efficacy of target gene knockdown. GAPDH is included as a loading control. (B) The analysis of tumor cell motility in vivo after delivery of control, integrin α3 or PKCα siRNA. Tumor-bearing embryos were treated with control IgG or mAB 1A5 (5μg) 1 day after tumor cell injection. Images of metastatic colonies were taken 2 days after antibody injection. The immobilization of tumor cells in response to mAB 1A5 was quantified in (C). N ≥ 5 with experiments performed in triplicate.