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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Clin Cancer Res. 2013 Oct 29;20(1):199–212. doi: 10.1158/1078-0432.CCR-13-0762

Figure 2. Azt/nimbolide arrests GBM cells at G1-S.

Figure 2

Flow cytometry using PI showing the effect of Azt/nimbolide on cell cycle of U87EGFRvIII cells (A-D), astrocytes (E-H), and Annexin V staining showing apoptosis of Azt-treated U87EGFRvIII cells (I) is shown. Effects of Azt on cell cycle of T98G cells (J-M) and U87 cells (N-Q) are also shown. Note that Azt treatment caused complete depletion of U87EGFRvIII cells from G2M at 12 hours and nearly 100% G1 arrest at 24 hours. Data is representative of three independent experiments. U87