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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cancer Res. 2013 Nov 6;74(1):363–373. doi: 10.1158/0008-5472.CAN-13-2016

Figure 1. Generation of 14-3-3ζ Transgenic Mice and Analysis of Mammary Tumorigenesis.

Figure 1

A, C, Schematic of the pWAP-HA-14-3-3ζ and pMMTV-HA-14-3-3ζ constructs. Arrows – genotyping primers locations. B, D, HA-14-3-3ζ protein IHC in lactating WAP-HA-14-3-3ζ and MMTV-HA-14-3-3ζ transgenic mice mammary glands (X400). E, H&E of lesions in multiparous wildtype and WAP-HA-14-3-3ζ mice (X400, both at 24 months of age) and MMTV-HA-14-3-3ζ tumor histology (X200, at 711 days old). F, MMTV-HA-14-3-3ζ mice (n=24) mammary tumor onset latency is shorter than wild-type mice (n=20). G, WAP-HA-14-3-3ζ mice (n=8) mammary tumor onset latency is shorter than wild-type (n=14) littermates following DMBA treatment.