Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cancer Res. 2013 Nov 6;74(1):363–373. doi: 10.1158/0008-5472.CAN-13-2016

Figure 5. MiRNA-221 expression is transcriptionally upregulated by 14-3-3ζ-mediated c-Jun activation.

Figure 5

A, Pri-miR-221, pre-miR-221 and mature miR-221 qRT-PCR analysis in MCF7-ζ versus control cells. B, Pri- and mature miR-221 qRT-PCR in MCF7 cells with 14-3-3ζ knockdown. C, IB analysis revealed increased JNK signaling and c-Jun expression in 14-3-3-ζ overexpressing cells versus control cells. D, Nucleo-cytoplasmic fractionation IB showed increased nuclear c-Jun localization in 14-3-3-ζ overexpressing cells. E, qRT-PCR analysis showed a decrease in pri-miR-221 expression with c-Jun knockdown (IB inset) in MCF7-ζ cells. F, ChIP analysis showed increased c-Jun binding to miR-221 promoter in MCF7-ζ cells versus control cells. G, Representative IHC of HA-14-3-3ζ, phospho-JNK, phospho-c-Jun, total-c-Jun, p27, Ki-67 and miR-221 ISH in MMTV-neu and MMTV-ζ.neu tumors (X400).