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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Clin Cancer Res. 2013 Sep 11;20(1):120–130. doi: 10.1158/1078-0432.CCR-13-0150

Figure 1. Nampt inhibition by FK866 or Nampt knockdown by siRNA reduces NAD levels in pancreatic cancer cells.

Figure 1

A, Different pancreatic cancer cell lines were imunoblotted for Nampt and Actin. B, NAD levels were measured in pancreatic cancer cells that were maintained in growth medium containing 1% FBS for 48 hours and then treated with FK866 or vehicle for 24 hours. C and D, Cells were transfected with Nampt siRNA or control siRNA analyzed by immunoblotting 72 hours later (insets). 24 hours after transfection cells were re-plated in growth medium containing 1% FBS and NAD levels were determined 48 hours later. * indicates p<0.05.