Figure 3. Nampt inhibition decreased pancreatic cancer cell survival and growth.

A, Cells were treated with vehicle or FK866 and submitted to MTT analysis 72 hours later. Values are means ± SD of three independent experiments. B, Cells were plated and treated with vehicle or FK866 24 hours later. Cells were counted by trypan blue dye exclusion assay 72 hours after treatment. C, Cells were grown in triplicates in soft agar containing different concentrations of FK866 for 7 days. Results represent the mean ± SD of three independent experiments. D, Cells were transfected with Nampt siRNA or a non-target siRNA (Control). 24 hours after transfection the cells were re-plated in growth medium containing 1% FBS and viable cells were counted by Trypan blue exclusion assay 48 hours later. Efficiency of knockdown was determined by immunoblotting. * indicates p<0.05