Figure 5. FK866 effect in pancreatic cancer cell viability is blocked by NMN and Nicotinic Acid, but not by SIRT1 or PARP-1 inhibitors.

A, PaTu8988t cells were treated with vehicle (control), 25 μm NMN or 25 μM Nicotinic Acid (NA) for 6 hours before addition of 2 nM FK866. Cells were submitted to MTT analysis 72 hours later. Values are mean ± SD of three independent experiments. Different pancreatic cell lines were imunoblotted for NAPRT1 and actin. B, PaTu8988t cells were treated with vehicle (control), 10 μM of the SIRT1 inhibitor (EX527) or 10 μM of the PARP inhibitor (4-Amino-1,8-naphthalimide) for 6 hours before addition of 2 nM FK866. Cells were submitted to MTT analysis 72 hours after treatment. Values are mean ± SD of three independent experiments. * indicates conditions that are significantly different than control, but are not significantly different from each other. C, Cells were transfected with siRNA specific for SIRT1 or a non-target siRNA. 24 hour after transfection the cells were re-plated and viable cells were counted at different time points. Values are mean ± SD of triplicates. Plot is representative of three independent experiments. D, PaTu8988t and SU86.86 cells were treated with the SIRT1 inhibitor EX527 (10 μM) or vehicle. Cells were counted 72 hours after treatment by trypan blue dye exclusion assay. Values are mean ± SD of three independent experiments.* indicates p < 0.05