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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Clin Cancer Res. 2013 Sep 11;20(1):120–130. doi: 10.1158/1078-0432.CCR-13-0150

Figure 6. Sensitivity of pancreatic cancer cells to FK866 depends on CD38 levels.

Figure 6

A, Different pancreatic cell lines were imunoblotted for CD38 and actin. B, PaTu8988t cells were transfected with CD38 siRNA or a non-targeting siRNA. 72 hours after transfection, we measured NAD levels and NADase activity. For MTT analysis, cells were re-plated 24 hours after transfection, and treated with vehicle control or FK866 24 hours later. MTT analysis was performed 48 hours after treatment. C, Panc-1 cells were transfected with a Flag-CD38 or empty vector. NAD levels and NADase activity were measured 72 hours later. For MTT analysis cells were re-plated 24 hours after transfection, and treated with vehicle control or FK866 24 hours later. MTT analysis was performed 48 hours after treatment. Efficiency of transfections was determined by immunoblotting 72 hours after transfection (insets). Values are mean ± SD of three independent experiments. * indicates p<0.05