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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cancer Res. 2013 Nov 12;74(1):243–252. doi: 10.1158/0008-5472.CAN-13-2245

Figure 2.

Figure 2

COX-1 is involved in neoplastic transformation. A, knockdown of COX-1 in JB6 CI41 cells was analyzed by Western blot. B, EGF- or TPA-induced cell transformation is dramatically reduced by COX-1 knockdown. JB6 cells were grown in soft agar in the absence or presence of EGF (10 ng/mL) or TPA (20 ng/mL) and colonies were counted as described in “Materials and Methods”. Data are presented as means ± S.E.M. from 3 independent experiments. The asterisks (***) indicate a significant (p < 0.001) difference compared to Mock group. C, knockdown of COX-1 partially blocks EGFR signal transduction in EGF-induced cell transformation. After starvation for 24 h, JB6 cells were stimulated with EGF (10 ng/mL) for 15 min. Cell lysates were subjected to Western blot analysis.