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(A) Schematic representation of hybrids. (B) Recombination of hybrid attP sites by Peaches- and Bxz2-Integrases. Int concentrations: 0, 125, 250, 500, 1000 nM. (C) Binding of Ints to hybrid attP. Int concentrations: 0, 125, 250, 500, 1000 nM. (D) Binding of CTDs to hybrid sites. CTD concentrations are as in Fig. 5B. Apparent affinities (Kd) are indicated.
