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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Angiogenesis. 2013 Oct 10;17(1):261–274. doi: 10.1007/s10456-013-9395-0

Fig. 4.

Fig. 4

SEMA4D induction of ANGPTL4 in HUVEC causes internalization of VE-cadherin. (a) Immunoblot for HUVECs transfected with scrambled oligos (C) or ANGPTL4 siRNA demonstrates successful suppression of ANGPTL4 protein (upper panel). GAPDH is used as the loading control (lower panel). (b) HUVEC monolayer, untreated, or treated with VEGF or sSEMA4D with or without ANGPTL4 siRNA, stained for VE-cadherin (green) and acid washed to remove cell surface/ membrane protein. HUVEC exhibit internalization of cadherin in VEGF or sSEMA4D compared to untreated controls, an effect lost in sSEMA4D treated cells with silenced ANGPTL4 (nuclei stained with DAPI, blue).