Table 2.
Effectiveness of lower doses of MEK1/2 inhibitor in dampening inflammationa from MO3.13 cells in the presence of B. burgdorferi (MOI 10:1) at 48 h of co-culture
| Inhibitor | CCL2 | CXCL8 | CXCL(1,2,3) | CCL5 | IL-6 |
|---|---|---|---|---|---|
| MEK 1/2 2.5 μM | 11.425 (± 2.665) | 8.625 (± 2.835) | 2.607 (± 0.563) | 1.732 (± 0.098) | 3.3385 (± 0.462) |
| MEK 1/2 5 μM | 13.66 (± 0.14) | 9.91 (± 2.04) | 3.04 (± 0.47) | 2.09 (± 0.45) | 4.765 (± 0.675) |
| MEK 1/2 10 μM | 18.1175 (± 2.648) | 6.385 (± 0.996) | 1.86 (± 0.148) | 2.1125 (± 0.239) | 5.9775 (± 0.978) |
a
Values represent average fold decrease in comparison to B. burgdorferi with solvent alone.
SEM value is indicated within parenthesis. The fold decrease in response to 10 μM MEK 1/2 inhibitor is the same as in Table 1. The average fold-change in response to the 2.5-μM and 5-μM doses was calculated from two independent experiments, while the 10-μM dose values were from four independent experiments