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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: J Immunol. 2013 Dec 11;192(2):676–682. doi: 10.4049/jimmunol.1301004

Figure 5. Combined IL-6 and IFNγ blockade further alleviates IRIS wasting and prolongs survival.

Figure 5

(A) Concentration of IFNγ in serum 10 days after injection of WT CD4 T cells into TCRα-/- mice harboring a chronic M. avium infection. Where indicated the mice were treated with IL-6 blocking mAb starting on day 0 of T cell transfer. The dotted line indicates the level of detection. Data are representative of 2 independent experiments. (B) WT CD4 T cells were injected into M. avium chronically infected TCRα-/- mice as before and treated with the indicated cytokine blocking antibodies. Weight change on day 14 after T cell transfer was measured. Data are pooled from 3 independent experiments. (C) After transferring CD4 T cells to induce IRIS as done in (A-B), mice were treated with a mixture of αIL-6+αIL-6R+αIFNγ mAbs either from day 0 to 16 or from 10-16 post-transfer of T cells and survival was monitored. Data are representative of two independent experiments. Treatment at day 0 and day 10 both significantly extended survival: P=0.001 for day 0 co-blockade group, and P=0.006 for day 10 blockade group compared to isotype control treated CD4 T cell recipients.