Table 1.
Upregulation of Differentially Expressed Genes in Aβ-Treated RPE Cells at 24 Hours
| Name | Description | GenBank Accession No. | Change (x-Fold) | P |
|---|---|---|---|---|
| Immune response | ||||
| IL-1β* | Homo sapiens interleukin 1, beta | NM_000576 | 4.80 | 0.0022 |
| RSAD2* | Homo sapiens radical S-adenosyl methionine domain containing 2 | NM_080657 | 2.58 | 0.0256 |
| IL-8* | Homo sapiens interleukin 8 | NM_000584 | 2.38 | 0.00177 |
| LAIR1 | Homo sapiens leukocyte-associated Ig-like receptor 1 | NM_021706 | 2.32 | 0.0332 |
| MX1* | Homo sapiens myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse) | NM_002462 | 2.18 | 0.0187 |
| OAS3 | Homo sapiens 2′-5′-oligoadenylate synthetase 3, 100kDa | NM_006187 | 1.99 | 0.000495 |
| IF144L* | Homo sapiens interferon-induced protein 44-like | NM_006820 | 1.98 | 0.00842 |
| IFI27* | Homo sapiens interferon, alpha-inducible protein 27 | NM_005532 | 1.81 | 0.00436 |
| OAS1 | Homo sapiens 2′,5′-oligoadenylate synthetase 1, 40/46kDa | NM_002534 | 1.80 | 0.0154 |
| PRKCZ | Homo sapiens mRNA, chromosome 1 specific transcript KIAA0505. | AB007974 | 1.71 | 0.0186 |
| CX3CL1 | Homo sapiens chemokine (C-X3-C motif) ligand 1 | NM_002996 | 1.71 | 0.00906 |
| LSP1 | lymphocyte specific protein 1 | W725T9 | 1.68 | 0.00255 |
| CXCL2* | Homo sapiens chemokine (C-X-C motif) ligand 2 | NM_002089 | 1.68 | 0.023 |
| TNFSF10* | Homo sapiens tumor necrosis factor (ligand) superfamily, member 10 (TRAIL) | NM_003810 | 1.58 | 0.0149 |
| OASL | Homo sapiens 2′-5′-oligoadenylate synthetase-like | NM_003733 | 1.57 | 0.016 |
| IL-33* | Homo sapiens chromosome 9 open reading frame 26 (NF-HEV) (C9orf26) | NM_033439 | 1.55 | 0.00679 |
| GBP4 | Homo sapiens guanylate binding protein 4 | NM_052941 | 1.55 | 0.0332 |
| MX2 | Homo sapiens myxovirus (influenza virus) resistance 2 (mouse) | NM_002463 | 1.54 | 0.0191 |
| CFI* | Homo sapiens I factor (complement) | NM_000204 | 1.54 | 0.0234 |
| SPN | Homo sapiens sialophorin (gpLI15, leukosialin, CD43) | NM_003123 | 1.50 | 0.0138 |
| Apoptosis | ||||
| XAF1* | Homo sapiens XIAP associated factor-1 | NM_017523 | 2.10 | 0.0192 |
| C8orf4 | Homo sapiens chromosome 8 open reading frame 4 | NM_020130 | 1.70 | 0.0152 |
| CECR2 | Homo sapiens cDNA FLJ34435 fis, clone HLUNG2000955 | AK091754 | 1.65 | 0.0156 |
| Transcription regulation | ||||
| ZNF175 | Homo sapiens zinc finger protein 175 | BC007778 | 1.92 | 0.00566 |
| THC2317006 | Q7IRG2(Q71RG2) FP2860, partial (26%) | THC2317006 | 1.89 | 0.0222 |
| FOXA2 | Homo sapiens forkhead box A2 | NM_021784 | 1.76 | 0.0107 |
| TEAD1 | Tea domain family member 1 (SV4O transcriptional enhancement factor) | CR594735 | 1.75 | 0.0169 |
| Transporter | ||||
| NR4A3* | Homo sapiens nuclear RNA export factor 3 | NM_022052 | 1.86 | 0.0198 |
| C14orf68 | Homo sapiens chromosome 14 open reading frame 68 | NM_207117 | 1.68 | 0.00185 |
| SLC30A4* | Homo sapiens solute carrier family 30 (zinc transporter), member 4 | NM_013309 | 1.64 | 0.0303 |
| NPCIL1 | Homo sapiens NPC1 (Niemann-Pick disease, type CI, gene)-like 1 | NM_013389 | 1.64 | 0.00187 |
| PKD2L2 | Homo sapiens polycystic kidney disease 2-like 2 | NM_014386 | 1.55 | 0.0118 |
| Cell proliferation | ||||
| NPY | Homo sapiens neuropeptide Y | NM_000905 | 2.65 | 0.0252 |
| MT3* | Homo sapiens metallothionein 3 (growth inhibitory factor (neurotrophic)) | NM_005954 | 1.71 | 0.0056 |
| Cell signaling | ||||
| STC1 | Homo sapiens stanniocalcin 1 | A1476245 | 1.99 | 4.14E-05 |
| TSHB | Homo sapiens thyroid stimulating hormone, beta | NM_000549 | 1.52 | 0.0498 |
| Metabolism | ||||
| CYP2D6* | Homo sapiens cytochrome P450, family 2, subfamily D, polypeptide 6 | NM_000106 | 1.82 | 0.000452 |
| SULT4A1 | Homo sapiens sulfotransferase family 4A, member 1 | NM_014351 | 2.02 | 0.00517 |
| Signal transduction | ||||
| PMCH | Homo sapiens promelanin-concentrating hormone | NM_002674 | 1.99 | 0.00127 |
| SSTR2* | Homo sapiens somatostatin receptor 2 | NM_001050 | 1.51 | 0.00651 |
| Miscellaneous function | ||||
| EGFL8 | Brain development: EGF-like-domain, multiple 8 | NM_030652 | 1.77 | 0.0248 |
| RCAN3 | Calcium-mediated signaling: RCAN family member 3 | BM457392 | 2.00 | 0.00402 |
| PACRG | Cell death: PARK2 co-regulated | NM_152410 | 1.55 | 0.0222 |
| FLCN | Cell cycle: folliculin | AL831885 | 1.50 | 0.0199 |
| FNDC5* | Extracellular matrix: fibronectin type III domain containing 5 | NM_153756 | 1.52 | 0.0299 |
| LOC221272 | Microtubule cytoskeleton organization: FLJ21659 fis, clone COL08743. | AK025312 | 1.64 | 0.0174 |
| FLJ21272 | Microtubule cytoskeleton organization: FLJ21272 fis, clone COL01753. | AK024925 | 1.56 | 0.0147 |
| MTMR9 | Phospholipid dephosphorylation: myotubularin related protein 9 | AF339813 | 1.55 | 0.0394 |
| DPP6 | Proteolysis: dipeptidyl-peptidase 6 | BX094874 | 1.54 | 0.0403 |
| PMCHL2 | Synaptic transmission: promelanin-concentrating hormone-like 2 | NM_153381 | 2.36 | 0.00696 |
| HERC5* | Ubiqitin cycle: hect domain and RLD 5 | NM_016323 | 1.73 | 0.000338 |
| CLDN6 | Cell-cell adhesion: claudin 6 | NM_021195 | 1.82 | 0.0277 |
| PRRG4 | Calcium-ion binding: proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) | NM_024081 | 2.19 | 0.00463 |
| SMOC1 | Calcium-ion binding: SPARC related modular calcium binding 1 | NM_022137 | 1.51 | 0.0104 |
| FRMD3 | Protein binding: FERM domain containing 3 | BG216229 | 1.59 | 0.0138 |
| LXN | Enzyme inhibitor: latexin | NM_020169 | 1.57 | 0.00212 |
| CMPK2 | Kinase: cytidine monophosphate(UMP-CMP) kinase 2, mitochondrial | BG547557 | 2.10 | 0.00714 |
| TRIM14 | Metal-ion binding: tripartite motif-containing 14 | NM_014788 | 1.53 | 0.0141 |
| DSCR6 | Protein binding: Down syndrome critical region gene 6 | NM_018962 | 1.87 | 0.0213 |
| CI6ORF70 | Protein binding: chromosome 16 open reading frame 70 | BC008476 | 1.56 | 0.0419 |
| AK123481 | Protein binding: K1AA1377 | AK123481 | 1.56 | 0.0034 |
| BRD4 | Protein binding: bromodomain containing 4 | NM_014299 | 1.64 | 0.0341 |
| RNASE1* | RNA binding: ribonuclease, RNase A family, 1 (pancreatic) | NM_198232 | 2.00 | 0.0026 |
The data are presented as x-fold changes and log-transformed x-fold changes. The expression level of genes greater than 1.5-fold was considered upregulated (P < 0.05).
*
Genes that are selected for verification by RT-PCR.