Table 3.
RT-PCR Primers
Gene | Forward Primer | Reverse Primer | Reference |
---|---|---|---|
IL-8 | AGGTGCAGTTTTGCCAAGGA | TTTCTGTGTTGGCGCAGTGT | |
IL-1β | AAGCTGAGGAAGATGCTG | ATCTACACTCTCCAGCTG | |
CFI | ATTGTGGAGACCAAAGTGATGAAC | TGATACTGGCTTGGAATGCAAA | |
CXCL2 | GATAGAGGCTGAGGAATCCAAGAA | ACATTTCCCTGCCGTCACA | |
RSAD2 | ACACAGGAATAATGACCCCAAAA | TGAGCTAAATGTCAGGTCCTGTGTA | |
MX1 | GCCAGGACCAGGTATACAG | GCCTGCGTCAGCCGTGC | Leong et al.32 |
IFI44L | CCAATTACACCTGAGCATTCTACTTTT | AGACATAAGCCACACAGTGAATCCT | |
IFI27 | TGGCTCTGCCGTAGTTTTGC | CACAGCCACAACTCCTCCAAT | |
HERC5 | GGGATGAAAGTGCTGAGGAG | CATTTTCTGAAGCGTCCACA | Kroismayr et al.33 |
SLC30A4 | GGCTATCATCAAAATCACCAACCA | CGGTGATGAGCATTATATCTCCATT | Overbeck et al.34 |
IL-33 | CACCCTCAAATGAATCAGG | GGAGCTCCACAGAGTGTTCC | Carriere et al.35 |
THSD2 | TGCACGAAGAAGGGAAAAACAT | TCTCGGACCCGTGTTTCAG | |
TNFSF10 | ATGGCTATGATGGAGGTCCAG | TTGTCCTGCATCTGCTTCAGC | Komatsuda et al.36 |
XAF1 | CCAGAAAATAAGTATTTCCACC | TTATACTTCTTGTCTTTGGACG | Kempkensteffen et al.37 |
CYP2D6 | CCTACGCTTCCAAAAGGCTTT | AGAGAACAGGTCAGCCACCACT | Kojima et al.38 |
SSTR2 | CCCCAGCCCTTAAAGGCATGT | GGTCTCCATTGAGGAGGGTCC | Kumar et al.39 |
NR4A3 | CAACTAGTCCAACCCCTTAAAATTCA | ACCTTGAGIAACTCTTCACCCTTCA | |
FN | GACCACACCGCCCACAAC | TCCTACATTCGGCGGGTATG | |
GAPDH | CATCCATGACAACTTTGGTATCGT | CAGTCTTCTGGGTGGCAGTGA | Wang et al.25 |
In total, 18 genes were selected for validation by RT-PCR. Nearly, all expression patterns were comparable to the microarray data.