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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Int J Cancer. 2013 Sep 30;134(6):1300–1310. doi: 10.1002/ijc.28465

Figure 3.

Figure 3

GTA induces G0 growth arrest of established glioma cell lines and primary tumor-derived GSCs in vitro. (a) Cell cycle profile of PI-labeled cells in growth/stem cell medium after 24 hours of 1 μM SAHA or 0.25% GTA treatment. GTA induced G0 growth arrest of all glioma cells, except U87, U251 and GBM8 GSCs, without affecting Oli-Neu OPCs or astrocytes and promoted neural stem cell (NSC) expansion. In contrast, SAHA significantly reduced proliferation of glioma and normal cells equally. (b) GSCs (50,000 cells per well of 24 well plate) were cultured in SCM in the absence or presence of 0.25% GTA or 1 μM SAHA for 5 days with medium replenished every 48 hours. While GTA-mediated growth reduction was largely cytostatic, SAHA-mediated growth reduction did not promote differentiation (except in GBM8 GSCs), but was more cytocidal. *p < 0.05, **p ≤ 0.01, #p ≤ 0.001, ##p < 0.0001. n ≥ 3 independent experiments. Scale bar = 200 μm.