Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Stem Cells. 2014 Jan;32(1):301–312. doi: 10.1002/stem.1528

Figure 3. γ Secretase inhibitors suppresses Notch signaling pathway in a dose- or time-dependent manner.

Figure 3

A) Western blotting was performed to analyze the cellular protein levels of Notch signaling pathway in 16 GICs. Activated Notch-1 (NICD) and Notch-1 pathway components Hes1, Hes3 and Hes5, were all expressed in the responder GICs. β-Actin was used as loading control. B) A panel of GICs was treated with the indicated doses of DAPT, BMS-708163 and RO4929097 for indicated time intervals. All the γ secretase inhibitors inhibited expression of NICD, Hes1, Hes3 and Hes5 in a time-dependent manner. The decrease in NICD was followed by a decrease in Hes1, Hes3 and Hes5 expression, whereas the expression of Notch-1 did not change. C) A panel of GICs was treated with the indicated doses of DAPT, BMS-708163 and RO4929097 for 48 hours. γ Secretase inhibitors inhibited expression of NICD, Hes1, Hes3 and Hes5 in a dose-dependent manner. D) Western blot analysis confirmed the knockdown effect of Notch shRNA lentivirus on GSC35. shRNA knockdown of Notch-1 in GICs decreased Hes1 and Hes5 protein expression.