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mESCs cultured in rice-derived rhLIF express alkaline phosphatase. mESCs cultured for 12 passages in rice-derived rhLIF or E. coli-derived recombinant mouse and human LIF were stained with alkaline phosphatase (Stemgent) following manufactures instructions and immediately photographed. Cells were grown in E. coli-derived recombinant mouse LIF (A), E. coli-derived recombinant human LIF (B), rice-derived recombinant human LIF (C), or without LIF (D).
