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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Sep 22;20(10):1214–1220. doi: 10.1038/nsmb.2664

Figure 2. mRNA–mRNA duplexes create SBSs and trigger SMD.

Figure 2

a, Diagram of FLUC-SOWAHC 3' UTR mRNA harboring 12 copies of MS2bs base-paired with CDCP1, TUFT1 or PPID mRNA via complementary 3' UTR Alu elements. b, Western blot (upper) or RT-sqPCR(lower) before (−) or after immunoprecipitation (IP) of lysates of formaldehyde-crosslinked HeLa cells that had been transiently transfected with pFlag-MS2-hMGFP and pFLUC-SOWAHC 3' UTR, pFLUC-SOWAHC 3' UTR-MS2bs or pFLUC-MS2bs. IP was performed using either anti-Flag or, as a control for nonspecific IP, mouse immunoglobulin G (mIgG). c, essentially as in b except pFLUC-SOWAHC 3' UTR-MS2bs or pFLUC-SOWAHC 3' UTR(ΔAlu)-MS2bs was used. d, essentially as in b except cells were transfected with Control or STAU1 siRNA. Error bars, s.e.m.; # of independently performed experiments = 3; P < 0.05 (one-tail t-test).