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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Sep 22;20(10):1214–1220. doi: 10.1038/nsmb.2664

Figure 5. Evidence that the SMD of mRNA–mRNA duplexes contributes to pancreatic adenocarcinoma cell migration and invasion.

Figure 5

a, RT-sqPCR and Western blotting (WB) analyses of lysates of BxPC3 cells that had been transiently transfected with the specified siRNA(s). GAPDH mRNA and Calnexin represent loading controls. b, Migration and invasion assays of BxPC3 cells after transfection with the specified siRNA(s). c, Model for the mechanism by which siRNA to SOWAHC mRNA, which encodes a protein of unknown function, promotes BxPC3-cell migration and invasion: downregulating SOWAHC mRNA inhibits SOWAHC mRNA-CDCP1 mRNA base-pairing and, thus, the SOWAHC mRNA-mediated SMD of CDCP1 mRNA, which encodes a protein that inhibits cell migration and invasion. # of independently performed experiments = 3, *, P <0.05 (one-tail t-test).