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. Author manuscript; available in PMC: 2014 Mar 9.
Published in final edited form as: Biochemistry. 2011 Feb 16;50(12):2170–2186. doi: 10.1021/bi101935x

Figure 1.

Figure 1

Analysis of protein purification by SDS-PAGE and western blot. (A) 10% SDS-PAGE gel stained with Coomassie Blue stain (B) western blot analysis using polyclonal antibody to the ACR4 kinase domain and (C) western blot analysis using monoclonal antibody to the NusA tag. Lane M, MW standards; lane 1, total protein from E. coli lysate; lane 2, affinity-enriched NusA:JKC; lane 3, NusA:JKC purified by gel filtration; lane 4, thrombin-cleaved NusA:JKC; lane 5, JKC purified by anion-exchange chromatography.