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The phosphorylation status of the inactive mutant (JKC2m), naïve JKC (JKC) and the autophosphorylated JKC (pJKC) determined by staining with Pro-Q Diamond (upper panel) and western blot using anti-phosphothreonine antibody (middle panel). The corresponding Coomassie Blue stained gel of the proteins is shown in the lower panel.
