Table 2.
Site-discriminating ions for phosphorylated residues (in red) in phosphopeptides. The percentage of maximum intensity for each site-discriminating ion in the averaged high resolution CID spectrum are shown in parenthesis (supporting ion information is available for underlined peptide sequences).
| Peptide | b ion | Phosphorylated b ion | y ion | Phosphorylated y ion |
|---|---|---|---|---|
| 471SSKDSAFTK479 | b4 (+1, 0.14) | b5 (+1, 27.6) | Y4 (+1, 1.14) | y5 (+2, 1.62) |
| 474DSAFTKDNGK483 | b4 (--) | b5 (+1, 4.9) | Y5 (+1, 10.7) | y6 (+2, 2.5) |
| 499VFTYEELEK507 | b2 (+1, 6.04) | b3 (+1, 5.77) | Y6 (+1, 12.82) | y7 (+1, 22.62) |
| 508AADGFKEESIVGK520 | b8 (+1, 0.77) | b9 (+1, 1.07) | Y4 (+1, 0.45) | y5 (+1, 0.54) |
| 508AADGFKEESIVGKGSFSCVYK528 | b14 (+2, 0.82) | b15 (+2, 3.92) | Y6 (+1, 6.07) | y7 (+1, 1.85) |
| 533DGTTVAVK540 | b2 (+1, 1.54) | b3 (+1, 1.34) | Y5 (+1, 097) | y6 (+2, 2.91) |
| 533DGTTVAVK540 | b3 (+1, 2.03) | b4 (--) | Y4 (+1, 3.51) | y5 (+1, 1.79) |
| 549QKNSNEFR556 | b3 (+1, 0.31) | b4 (--) | Y4 (+2, 2.50) | y5 (+2, 1.46) |
| 551NSNEFRTELDLLSR564 | b6 (+1, 0.71) | b7 (+1, 2.59) | Y7 (+1, 3.33) | y8 (+1, 0.55) |
| 557TELDLLSR564 | b6 (+1, 3.16) | b7 (+1, 3.64) | Y1 (--) | y2 (--) |
| 641DIKSSNILIDEEHNAR656 | b3 (+1, 2.82) | b4 (+1, 2.63) | Y12 (+3, 8.75) | y13 (+2, 26.46) |
| 641DIKSSNILIDEEHNAR656 | b4 (+1, 3.62) | b5 (--) | Y11 (+2, 9.37) | y12 (+2, 6.06) |
| 826SGSENTEFR834 | b5 (+1, 2.38) | b6 (+1, 0.42) | Y3 (+1, 5.06) | y4 (+1, 5.44) |
| 835GGSWITFPSVTSSQR849 | b2 (--) | b3 (--) | Y12 (+2, 5.39) | y13 (+2, 4.55) |
| 875SLEEEIGPASPGQSLFLHHNF895 | (--) | b1 (--) | Y20 (+2, 0.15) | y21 (--) |
| 875SLEEEIGPASPGQSLFLHHNF895 | b13 (--) | b14 (+1, 0.32) | y7 (+1, 0.48) | y8 (--) |