Table I.
Induction of microRNAs in human prostate tumors
| miRNA |
Gleason score |
Hypoxia-inducedb LNCap cells | REST-knockdownc NC cells | |||
|---|---|---|---|---|---|---|
| 3a | 5a | 7a | 8-10a | |||
| miR-21 | 1.22 ± 0.45 | 1.50 ± 0.15 | 2.50 ± 0.22 | 5.05 ± 0.72 |
|
|
| miR-24 | 0.90 ± 0.36 | 1.36 ± 0.56 | 2.06 ± 0.42 | 13.4 ± 1.18 | ||
| miR-25 | 0.53 ± 0.22 | 1.03 ± 0.46 | 1.96 ± 0.20 | 11.6 ± 1.02 | ||
| miR-30e | 1.37 ± 0.15 | 1.22 ± 0.08 | 2.83 ± 0.09 | 19.0 ± .090 | ||
| miR-125b | 1.22 ± 0.08 | 1.77 ± 0.43 | 2.01 ± 0.15 | 6.70 ± 0.85 | ||
| miR-142-3p | 1.26 ± 0.14 | 1.92 ± 0.26 | 3.78 ± 0.48 | 7.63 ± 0.39 | ||
| miR-151-5p | 0.11 ± 0.02 | 0.78 ± 0.30 | 4.38 ± 0.54 | 10.3 ± 1.35 | ||
| miR-210 | 0.01 ± 0.00 | 1.88 ± 0.30 | 2.25 ± 0.18 | 4.60 ± 0.48 | ||
| miR-223 | 2.28 ± 0.88 | 1.42 ± 0.06 | 1.35 ± 0.13 | 10.8 ± 0.44 | ||
| miR-374a | 0.24 ± 0.10 | 0.80 ± 0.40 | 1.16 ± 0.22 | 20.8 ± 1.48 | ||
| miR-9 | 0.65 ± 0.13 | 1.21 ± 0.35 | 1.74 ± 0.14 | 3.89 ± 0.55 |
|
|
| let-7e | 0.09 ± 0.03 | 1.02 ± 0.12 | 3.46 ± 0.36 | 14.3 ± 0.68 | ||
| miR-302b | 0.01 ± 0.01 | 1.35 ± 0.77 | 3.14 ± 0.86 | 7.63 ± 1.25 | ||
| miR-19b | 0.37 ± 0.11 | 1.41 ± 0.14 | 1.74 ± 0.34 | 4.76 ± 0.84 | ||
| miR-29c | 0.27 ± 0.03 | 1.31 ± 0.07 | 1.44 ± 0.09 | 3.37 ± 0.07 | ||
| miR-30d | 1.51 ± 0.31 | 2.06 ± 0.71 | 2.65 ± 0.71 | 22.7 ± 1.87 | ||
| let-7g | 0.07 ± 0.02 | 0.91 ± 0.06 | 3.58 ± 0.88 | 3.41 ± 0.93 | ||
| miR-30c | 0.37 ± 0.19 | 0.86 ± 0.37 | 2.25 ± 0.41 | 3.74 ± 1.14 |
|
|
| miR-7 | 0.27 ± 0.14 | 1.27 ± 0.10 | 3.36 ± 0.54 | 3.51 ± 1.12 | ||
| miR-27a | 1.59 ± 0.42 | 2.13 ± 0.46 | 2.28 ± 0.06 | 15.9 ± 1.00 | ||
| miR-106b | 0.80 ± 0.14 | 1.73 ± 0.54 | 1.93 ± 0.44 | 2.49 ± 0.54 | ||
| miR-150 | 1.03 ± 0.17 | 1.12 ± 0.04 | 1.65 ± 0.15 | 7.27 ± 0.40 | ||
| miR-155 | 1.42 ± 0.24 | 1.47 ± 0.13 | 1.45 ± 0.13 | 5.58 ± 0.16 | ||
| miR-93 | 0.02 ± 0.02 | 1.87 ± 1.04 | 1.84 ± 0.48 | 2.50 ± 0.22 | ||
| miR-191 | 0.38 ± 0.21 | 1.43 ± 0.31 | 0.91 ± 0.31 | 6.28 ± 1.48 | ||
| miR-424 | 0.10 ± 0.04 | 1.04 ± 0.36 | 1.67 ± 0.11 | 8.28 ± 0.12 | ||
| miR-425 | 1.46 ± 0.54 | 1.22 ± 0.62 | 3.48 ± 0.24 | 4.06 ± 0.94 | ||
The human miFinder RT2 PCR array (Qiagen), composed of the most abundant and best characterized miRNAs, was employed for miRNA expression in RNA samples isolated from human prostate tumors with Gleason scores ranging from 3 to 10. Specifically, RNA from at least five tumors per group, Gleason scores 5 through 10, were pooled for the miRNA PCR array analysis. Pooled RNA from the corresponding benign prostate tissues served as noncancerous control. Results represent the average of three independent RNA preparations +/- standard error of the mean.
miRNA expression employing the human miFinder RT2 PCR array (Qiagen), in RNA isolated from LNCaP cells grown in normoxia vs. hypoxia for three days (Supplementary Table S3).
miRNA expression employing the human miFinder RT2 PCR array (Qiagen), in RNA samples isolated from NC cultures following REST-knockdown by REST siRNA transfection vs. transfection with scrambled siRNA (Supplementary Table S4).