Figure 3. Enhanced OSIC self-renewal and tumorigenicity induce a progressive expansion of CD49f+ cells forming the osteosarcoma mass.
(A-C) Expression levels of CD59, CD28, CD43, CD117, CD133 and STRO-1 (panel A) as well as CD49f (panels B, C) were determined by FACS in U2OS, UT2 and UT3 cells. (D) FACS analysis of CD49f expression in normal (MSC), KHOS/NP, and 8 different primary osteosarcoma cells. (E & F) Inverse relationship between CD49f levels vs. time for tumor formation in serially transplanted (panel E) and the first transplanted cells (panel F).