Figure 7. The OSIC-like CD49f−CD133+ phenotype identifies a small fraction of transformed cells in patient samples of either primary or metastatic osteosarcoma.
(A) FACS analysis of CD49f−CD133+ versus CD49f+ cells in TTC444 cells. Ab, antibody. (B-D) HE staining of the paired tissue samples from both primary and concordant metastatic pulmonary sites in 3 osteosarcoma patients. Parallel immunohistochemical assay detected the expression of CD49f, CD133, and CXCR4 in these samples.