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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Traffic. 2013 Mar 11;14(6):709–724. doi: 10.1111/tra.12059

Figure 3. Depletion of flotillin-1 and flotillin-2 does not inhibit PMA-dependent endocytosis of DAT in HEK/DAT cells.

Figure 3

HEK/DAT cells were transfected with non-targeting siRNA (NT), SmartPool™ (SP) to flotillin-2 (B), Duplex 6 (D6) (A) or together flotillin-1 (D6) and flotillin-2 (SP) (B). Cells were treated with 1 μM PMA or DMSO (veh) for 30 min. The cells were fixed, permeabilized and stained with rat monoclonal antibody to DAT (red) and rabbit antibodies to flotillin-1 (A) or flotillin-2, or both flotillins together (B). Representative images (single confocal sections) of 3D stack of confocal images are presented. Identical intensity scales are used and shown on the images. Scale bars, 10 μM.