Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Traffic. 2013 Mar 11;14(6):709–724. doi: 10.1111/tra.12059

Figure 5. Flotillin depletion increases diffusion mobility rates of CFP-HA-DAT.

Figure 5

HEK/CFP-HA-DAT cells were transfected with NT siRNA, siRNAs to flotillin-1 alone or together with flotillin-2 siRNA as in Figure 2. Average depletion of flotillin-1 was 72.8% and 85,2% in single and double siRNA transfected cells. FRAP experiments were performed as described in “Methods”.

(A) Examples of images of the CFP fluorescence with focusing on cell edges and on areas of diffuse fluorescence acquired before (-1 s), immediately after (0 s) and 5 sec after photobleaching of the indicated areas.

(B) Bar graphs represent mean values of τ and Mf from multiple cells in 3 experiments. S.E. (statistical error); n=16–20. *P<0.05; **P<0.005.