Primary mesencephalic postnatal cultures were fixed, permeabilized and stained with rat monoclonal antibody to DAT (green) and rabbit monoclonal antibody to flotillin-1 (red) followed by secondary to rat and rabbit labeled with Alexa488 and Cy5, respectively. Left column, soma and proximal processes. Right column, distal processes. Insets below show high magnification 3-D volume view of areas marked by white rectangles. Scale bars, 10 μM.