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. Author manuscript; available in PMC: 2014 Mar 9.
Published in final edited form as: Neuroscience. 2007 Aug 2;149(4):822–833. doi: 10.1016/j.neuroscience.2007.06.060

Figure 1. Constituents of the apoptosome Complex accumulate and caspases 9 and 3 are activated following neonatal HI in soluble fraction from injured rat forebrain.

Figure 1

A. Immunoblots showing increased levels of both APAF 1 and cytochrome c and the presence of caspase-9 in soluble protein extracts from ipsilateral injured forebrain following neonatal HI in comparison to uninjured controls. B. Increased biochemical activity of caspases 9 and 3 (* p≤ 0.05 vs control, ANOVA with Dunnett’s post hoc test) after HI. C. The active p17 cleaved fragment of caspase-3 is found with increasing abundance at 6 and 24 hours after HI compared to control. Commassie stained gels or ponceau stained nitrocellulose membranes are shown as loading controls for each of the immunoblots in A and C.