Figure 2. Anti–hDEC-pdA:dT activates human MoDCs.

(a) ELISA analysis of the production of IFN-α by MoDCs treated overnight with various doses of pdA:dT (with or without 1 µg ml⁻¹ lipofectamine), anti–hDEC-pdA:dT and hControl Ig-pdA:dT. Error bars represent the mean ± s.d. of three independent experiments, each performed in triplicate. (b) Time course of the production of IFN-β by MoDCs treated with anti–hDEC-pdA:dT (5 µg ml⁻¹) as determined by ELISA. Histograms represent mean ± s.d. (n = 3). (c) MoDCs were pretreated for 2 h with 10 µg of various blocking mAbs before adding 1 µg ml⁻¹ or 5 µg ml⁻¹ of anti–hDEC-pdA:dT. IFN-α production was monitored by ELISA and is shown as the mean ± s.d. of three experiments performed in triplicate per condition.