Fig. 3. Impaired osteogenic potential in ESET-deficient mesenchymal stem cells.
a, mesenchymal stem cells isolated from the bone barrow of one month-old wild-type and (exon 15&16)CKO/CKO littermate were cultured in osteogenic medium for two weeks, then stained for alkaline phosphatase activity (red). b, mesenchymal stem cells from one month-old wild-type and (exon 15&16)CKO/CKO littermate were cultured in osteogenic medium for three weeks, then stained with alizarin red for detection of calcium deposit (red). Representative areas in the wells are enlarged for more details in the corresponding lower panels. Scale bar: 400 μm.
