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. Author manuscript; available in PMC: 2014 Dec 11.
Published in final edited form as: FEBS Lett. 2013 Nov 1;587(24):3961–3967. doi: 10.1016/j.febslet.2013.10.028

Fig. 4. Repression of Runx2-mediated reporter gene activation by ESET.

Fig. 4

a, Diagram of the mOG2-Luc reporter used in transfection of C3H10T1/2 cells is shown at the top. Total amount of DNA in each transfection was kept constant by the addition of empty vector DNA, and asterisks indicate significance of luciferase reporter activities between plasmid combinations (*p=0.004; **p<0.001; ***p=0.003). b, Diagram of the doxycycline (Dox)-inducible siRNA construct is shown on the top. Time-dependent siRNA knockdown of ESET was assessed by western blotting with an anti-ESET antibody. c, ATDC5 cells harboring Dox-inducible control siRNA vector (columns 1-2) or Dox-inducible specific siRNA vector (columns 3-4) were transfected with the Runx2-responsive mOG2-Luc reporter. Note that cells in columns 2 and 4 were already cultured in Dox medium for at least 7 days prior to transfection, and kept in Dox medium after transfection. Asterisks indicate significance of luciferase reporter activities between cells cultured in medium with or without Dox (*p=0.65; **p<0.01).