Skip to main content
. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: Neuroscience. 2013 Nov 6;257:11–19. doi: 10.1016/j.neuroscience.2013.10.058

Figure 4. IL-1β primed neurons are more vulnerable to proNGF than NGF.

Figure 4

A. Immunostaining of hippocampal neurons for sortilin and p75NTR demonstrating colocalization of the receptors. B. Bars show relative cell number (mean±SEM, n = 4 independent experiments) in hippocampal neuronal cultures. Cells were treated for 4–6 h with IL-1β (10ng/mL), followed by overnight treatment with NGF (100ng/mL). IL-1β did not exacerbate NGF-mediated neuronal death. Asterisks denote difference from untreated control (p < 0.05). C. Bars indicate relative cell number (mean±SEM, n = 4 independent experiments) in hippocampal neuronal cultures treated with IL-1β (10 ng/mL) for 4–6 hrs, followed by proNGF (1–10ng/mL). ProNGF elicited more cell death in IL-1β primed neurons. * denotes difference from control and # indicates difference from proNGF alone (p<0.05; one-way ANOVA and Tukey’s post hoc analysis).