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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: Neuroscience. 2013 Nov 6;257:11–19. doi: 10.1016/j.neuroscience.2013.10.058

Figure 5. IL-1β recruits sortilin and p75NTR receptors to the plasma membrane.

Figure 5

A. Cultured hippocampal neurons were treated with IL-1β for 8h, incubated with biotin for 1h, and lysates were precipitated with streptavidin. Total biotinylated cell surface protein and 20μg of non-biotinylated intracellular proteins were analyzed by Western blot for sortilin, p75NTR and transferrin receptor. Blots were stripped and re-probed for actin, which was only present in the intracellular fraction. Quantification of sortilin (B) and p75NTR (C) from three independent blots. Asterisk denotes difference from control (p<0.05; one-way ANOVA and Tukey’s post hoc analysis).