Figure 4.
Morphology of the collagen assemblies formed in the presence of the anti-α2Ct scFv construct added at indicated scFv:collagen I molar ratios. As indicated by the punctate staining seen in panels A and B, at the highest scFv:collagen I molar ratios, the formation of properly folded spindle-shaped collagen fibrils is inhibited. In contrast, at the relatively low concentrations of inhibitory scFv, abundant spindle-shaped collagen fibrils are formed (D, E). Panels C and F depict the ultrastructure of assemblies formed at high (C) and low (F) scFv: collagen I molar ratios. Arrows indicate fibrils with pointed and blunt ends.